Molecular Biology for Pathology Technicians

Molecular biology is a discipline that has been rapidly developing and implementing in Pathology services in recent years. In the era of Precision Oncology, the study of genetic biomarkers acquires a great impact on the clinical management of patients from a diagnostic, prognostic and therapeutic point of view. At present, it is of great importance, above all due to the increasing incorporation of oncological treatments directed against specific molecular targets, which has led to the implementation of these analyzes in the therapeutic guidelines of some of the most prevalent tumors, such as lung cancer or cancer. colorectal cancer. Furthermore, the molecular detection of human papillomavirus (HPV) currently represents a new approach for the screening and prevention of cervical cancer.

 

This course covers the main aspects to be known by the Pathological Anatomy technician for the understanding and updating of: the characteristics and properties of nucleic acids (DNA - and circulating free DNA or liquid biopsy - and RNA) , the optimization of its obtaining from the different types of samples common in Pathological Anatomy laboratories, the different types of genetic alterations, the most used techniques for their study in the Molecular Biology laboratory and the application of these for detection of biomarkers. Thus, this course addresses, in a global and updated way, the theoretical and applied aspects that the Pathological Anatomy technician requires to develop their work in the Molecular Biology laboratory.

 

Who is it addressed to:

Pathological Anatomy Technicians, Biologists and Pathological Anatomy Resident Physicians.

* Continuing education credits are not valid for resident physicians.

Valid event with the FENIN Code of Ethics

 

School periods available for enrollment:

2nd edition: from December 1, 2021 to February 27, 2022
3rd edition: from March 1, 2022 to May 29, 2022
4th edition: from June 1, 2022 to August 28, 2022

 

 

Topic 1. Introduction to nucleic acids.

 

1.1 Introduction: from gene to protein.

Exons and introns, process from DNA to protein, genetic code.

Rubén Carrera

1.2 Introduction to nucleic acids: DNA and RNA .

Composition, structure and properties of DNA and RNA. DNA replication as an introduction to PCR.

Miriam Sansó

1.3 Circulating free DNA.

Definition, characteristics and applications.

Dra Beatriz Bellosillo

 

Topic 2. Extraction of nucleic acids and quality control

 

2.1 Pre-analytical considerations

Macro / microdissection of paraffin tissue. Characteristics of the different types of sample (paraffin biopsies, cytologies, circulating free DNA and fresh samples) as a source of nucleic acids for molecular study.

Javier Hernández

2.2 Nucleic acid extraction and purification methods.

Manual / automated extraction from different sample types. Conservation of nucleic acids.

Javier Hernández

2.3 Nucleic acid quantification systems.

Spectrophotometry and fluorometry. Fundamentals and assessment of the purity and quality of the nucleic acids obtained.

Leyla Blanco

2.4 Nucleic acid electrophoresis

Gel and automated electrophoresis. Utilities in assessing the quality of DNA and RNA.

Yolanda Ruano

 

Topic 3. Genetic variants.

 

3.1 DNA alterations.

Mutations, definition and types. Chromosomal alterations: alteration in copy number, gains and losses.

Emma Borràs

3.2 Alterations in the RNA.

Rearrangements and fusion genes.

Emma Borràs

3.3 Classification and interpretation of variants in cancer.

Somatic and germline variants. Pathogenicity and actionability.

Conchi Fernández

 

Topic 4. Analysis techniques in the molecular biology laboratory

 

4.1 Introduction to the polymerase chain reaction (PCR).

Fundamentals, components and stages. PCR from RNA: RT-PCR.

Maria Garrido

4.2 Qualitative PCR.

Fundamentals and applications in pathological anatomy.

Yolanda Ruano

4.3 PCR in real time.

Fundamentals and applications in pathological anatomy.

Michele Biscuola

4.4 digital PCR.

Fundamentals and applications in pathological anatomy.

Miriam Sansó

4.5 Fragment analysis and Sanger sequencing.

Foundations and applications in pathological anatomy. Michele Biscuola

4.6.1 Next Generation Sequencing I.

Introduction, preanalytical considerations and overview of the process.

Pedro Jares

4.6.2 Next Generation Sequencing II.

Technical fundamentals, amplicon enrichment vs capture. Applications in pathological anatomy.

Pedro Jares

4.7 OSNA® system

Fundamentals and applications in pathological anatomy.

Elena Gonzalvo

4.8 nCounter® system

Fundamentals and applications in pathological anatomy.

Cristina Teixidó

4.9 Determination techniques Human Papillomavirus (HPV)

Fundamentals and applications in pathological anatomy.

Mercè Muset

 

Topic 5. Study of molecular biomarkers in cancer

 

5.1 Introduction to biomarkers molecular.

Biomarker definition. Types and implication in the diagnosis, prognosis and treatment of cancer.

José Antonio López-Guerrero

5.2 Molecular biomarkers in lung cancer.

What are they, clinical relevance and main detection techniques.

Sergi Clavé

 

5.1 Molecular biomarkers in colorectal cancer.

What are they, clinical relevance and main detection techniques.

Noelia Tarazona

5.2 Molecular biomarkers in breast and ovarian cancer.

What are they, clinical relevance and main detection techniques.

Sergi Clavé

5.3 Molecular biomarkers in stromal tumors.

What are they, clinical relevance and main detection techniques.

José Antonio López-Guerrero

5.4 Human Papillomavirus (HPV).

Types and clinical relevance.

Belen Lloveras

SEAP
Teaching Hours
33
Price
75 €
Credits

Solicitada la acreditación a la Comisión de Formación Continuada de las Profesiones Sanitarias de la Comunidad de Madrid.

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Management

Dra. Carolina Martínez Ciarpaglini

Dra. Carolina Martínez Ciarpaglini

Médica Patóloga Adjunta al Servicio de Anatomía Patológica
HU de Valencia
Nº Colegiado: 464622652
Colegio de Médicos de Valencia

Coordination

Dra. Conchi Fernández Rodríguez

Dra. Conchi Fernández Rodríguez

Bióloga Adjunta en el Laboratorio de Diagnóstico Molecular
Servicio de Patología del Hospital del Mar (Barcelona)
Nº Colegiado: 22278-C
Colegio de Biólogos de Cataluña